Dr. Michelle Gumz’ Lab
Dr. Gumz has a long-time interest in the molecular control of renal function. The Gumz laboratory is investigating the role of the circadian clock in the kidney. The focus is on the regulation of sodium transport in the kidney, which is critical to the control of blood pressure as well as to overall cardiovascular health. Aldosterone is a mineralocorticoid hormone that regulates sodium balance and blood pressure. As a graduate student, Dr. Gumz was the first to identify the circadian clock gene Period 1 as an aldosterone target. She has subsequently shown that Period 1 regulates transcription of alpha ENaC, the aldosterone-regulated and rate-limiting subunit of the epithelial sodium channel. Techniques commonly employed in the lab include microarray studies, real time PCR analysis, Western blotting, and analysis of DNA/protein interactions. In vitro experiments are routinely performed using several cell culture models of the renal collecting duct. Future experiments will utilize electrophysiology to measure sodium transport in these cell lines. In vivowork is done using mouse models and upcoming studies include the use of radio-telemetry to evaluate blood pressure in Period 1 knockout mice. The Gumz laboratory continues to identify novel Period 1 targets and determine how these targets contribute to the regulation of sodium transport in the kidney.
A complete listing of Dr. Gumz’ scientific journal publications are available here.
University of Florida Research Landscapes: Dr. Gumz Lab