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Division of Nephrology, Hypertension & Renal Transplantation Department of Medicine in the College of Medicine
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Education Overview

Pitfalls in Light Microscopic Immunolocalization, Jill Verlander (W-0003)

You can watch the video lecture using the link below. After watching the video, return to this page to take the quiz and survey for credit.

https://youtu.be/tjD8RGxkd5Q

YouTube CME Channel

Presented by
Jill Verlander, DVM
Scientist, Director of College of Medicine Core Electron Microscopy Lab
UF Division of Nephrology, Hypertension & Renal Transplantation

Target Audience
Medicine faculty, fellows, residents, researchers

Program Description
This CME activity consists of an educational component (slides, audio lecture) in an electronic format, followed by an online post-test. Estimated time to complete this activity, including review of materials, is 1.5 hours.

Requirements for Successful Completion

  • This CME activity consists of an educational component (audio lecture in an electronic format, which is followed by an online post-test).
  • Certificates are awarded upon successful completion (80% proficiency) of the post-test.
  • In order to receive credit, participants must view the presentation in its entirety.
  • The University of Florida College of Medicine will report CME credit to CE Broker when applicable.
  • There is no fee to participate in this CME activity or to receive CME credit.

Learning Objectives
As a result of participation in this activity, participants should be able to:

  • Recognize limitations inherent in light microscopic
  • Practice light microscopic immunolocalization
  • Locate problems that can lead to mistaken interpretation of results

Faculty Disclosure
Jill Verlander has disclosed that she has no relevant financial disclosures. No one else in a position to control content has any financial relationships to disclose.

Continuing Medical Education Credit
Accreditation: The University of Florida College of Medicine is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.

Credit: The University of Florida College of Medicine designates this enduring material for a maximum of 1 AMA PRA Category 1 Credit™. Physicians should claim only the credit commensurate with the extent of their participation in the activity.

Release Date: December 30, 2015

Expiration Date: Decenmber 30, 2016

CME Course Credit Registration W-0003

Name(Required)
Address(Required)

Quiz

You must score 80% in order to receive a passing grade and CME credit for the course.
1. If an antibody against the correct sequence for a protein does not react with tissue sections, it means the protein is not present in the tissue.(Required)
2. If an antibody localizes well in one cell type in a tissue, but the remainder are negative, it means the target protein is present in only the one cell type that was positive.(Required)
3. In tissues with low target antigen abundance, the detection may not be improved by:(Required)
4. Thinner tissue sections produce greater immunoreactivity than thicker sections.(Required)
5. Thinner tissue sections produce better resolution than thicker sections.(Required)
6. By light microscopy, stronger, more distinct immunoreactivity along the apical limit of cells indicates increased apical plasma membrane antigen localization.(Required)
7. The best resolution available to determine subcellular immunolocalization of a protein is(Required)
8. Immunohistochemistry results should correlate with the results of other methods, such as immunoblot, RT-PCR, and physiology.(Required)
9. Examples of antigen retrieval methods that can be used to "unmask" tissue antigens does not include(Required)
10. Specific antibodies raised against different sub-units of a protein complex, such as the vacuolar proton pump:(Required)

Please fill out the CME Evaluation Survey upon completion of this course.

You will receive a confirmation email with additional information approving your course credit.